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Jana U'Ren, Lilly Moore 2021. Inoculating and harvesting fungal isolates on cellophane overlay . protocols.io https://dx.doi.org/10.17504/protocols.io.qtedwjeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.qtedwje
Authors: Jana U'Ren, Lilly Moore
Group: U'Ren lab - Ecosystem Genomics
Summary: Some fungal isolates will embed into the solid media during growth, which makes it difficult to harvest agar-free mycelium for downstream applications such as DNA and RNA extraction. Here, we describe a protocol to (1) inoculate fungal isolates onto solid media with sterile cellophane overlays and (2) harvest the tissue for later DNA or RNA extraction.
Affiliations: University of Arizona, University of Arizona
Version: 1
Publication Date: 2021
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