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Jessica J. Miciak, Jason Hirshberg and Fred Bunz, Fred Bunz 2018. AdenoBuilder: modular assembly of recombinant adenovirus genomes from high-copy plasmids. protocols.io https://dx.doi.org/10.17504/protocols.io.qt5dwq6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.qt5dwq6
Authors: Jessica J. Miciak, Jason Hirshberg and Fred Bunz, Fred Bunz
Summary: The diverse applications of mutant and recombinant adenoviruses demand facile methods for their generation. This protocol allows the user to rapidly assemble customized adenoviral genomes from high-copy plasmids in a two-step isothermal reaction. Following in vitro assembly, full-length genomes can be directly packaged in HEK293 cells. Crude preparations of infectious virus can be prepared in less than one week.
Affiliations: Johns Hopkins University, Johns Hopkins University, Johns Hopkins University
Version: 1
Publication Date: 2018
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