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URL: https://dx.doi.org/DOI:10.17504/protocols.io.q9edz3e

Authors: Helle Glud Binderup, Jonna Skov Madsen, Kim Houlind, Rikke Fredslund Andersen, Claus Lohman Brasen

Summary: A protocol for quantification of circulating microRNA using TaqMan assays.MicroRNAs are purified from platelet-poor plasma using Nucleospin columnsDuring the purification step samples are spiked with cel-miR-39 as a mean of technical normalizationNormalization is performed using the ΔCt-method

Associated Publications: Binderup HG, Madsen JS, Heegaard NHH, Houlind K, Andersen RF, Brasen CL (2018) Quantification of microRNA levels in plasma – Impact of preanalytical and analytical conditions. PLoS ONE 13(7): e0201069. doi: 10.1371/journal.pone.0201069

Affiliations: Biochemistry and Immunology, Lillebaelt Hospital, Kolding and Vejle, Denmark, Biochemistry and Immunology, Lillebaelt Hospital, Kolding and Vejle, Denmark, Department of Vascular Surgery, Lillebaelt Hospital, Kolding, Denmark, Biochemistry and Immunology, Lillebaelt Hospital, Kolding and Vejle, Denmark, Biochemistry and Immunology, Lillebaelt Hospital, Kolding and Vejle, Denmark

External URL: https://doi.org/10.1371/journal.pone.0201069

Version: 1

Publication Date: 2018

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