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Etienne Laliberté, Raymond Soffer 2018. Measuring spectral reflectance and transmittance (350-2500 nm) of large leaves using the Spectra Vista Corporation (SVC) DC-R/T Integrating Sphere. protocols.io https://dx.doi.org/10.17504/protocols.io.p8pdrvnCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.p8pdrvn
Authors: Etienne Laliberté, Raymond Soffer
Group: Canadian Airborne Biodiversity Observatory
Summary: Here we describe the standardised protocol used by the Canadian Airborne Biodiversity Observatory (CABO) to measure leaf spectral reflectance and transmittance, using the Spectra Vista Corporation (SVC) DC-R/T Integrating Sphere fitted to a portable full-range SVC HR-1024i field spectroradiometer. This standard version of our protocol describes the common case where an individual leaf is large enough to entirely cover the reflectance or transmission port of the integrating sphere. Briefly, six mature, healthy-looking and sunlit leaves from a canopy plant are selected for measurements of adaxial reflectance and transmittance. Reflectance measurements are referenced to a calibrated Spectralon® disk and corrected for stray light. Our leaf spectroscopy protocol builds from that of the Carnegie Airborne Observatory.
Affiliations: Université de Montréal, National Research Council
Version: 5
Publication Date: 2018
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