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URL: https://dx.doi.org/DOI:10.17504/protocols.io.nz4df8w

Authors: Ian Mackay

Summary: This is an excellent rhinovirus screening assay which I have used on thousands of sample extracts mostly originating from acutely ill paediatric patients, spanning well over a decade's worth of collection dates.I have not confirmed that it can detect every single genotype but I do know that it detects many from each of the three RV species (Human rhinovirus A, Human rhinovirus B and Human rhinovirus C) as well as at least some Human enterovirus genotypes.The assay does pick up some enteroviruses due to their genetic similarities in the 5'UTR target region. These can be discriminated using subgenomic sequencing (see  VP42 typing assay protocol), or simply described as "respiratory enteroviruses" since there is no specific-specific vaccine or treatment available anyway. The assay was created and first described in 2008 by Xiaoyan Lu and colleagues at the US CDC [Ref 1]. In collaboration with Lu et al., we published a small update that included Lu's modified forward primer design. We routinely use it with this primer (hence the 'mod' text added to the primer name) but without the expensive special base, as outlined in Step 1.  

Affiliations: The University of Queensland

Version: 1

Publication Date: 2018

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