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URL: https://dx.doi.org/DOI:10.17504/protocols.io.nuudeww

Authors: J. Greg Caporaso, Gail Ackermann, Amy Apprill, Markus Bauer, Donna Berg-Lyons, Jason Betley, Noah Fierer, Louise Fraser, Jed A. Fuhrman, Jack A. Gilbert, Niall Gormley, Greg Humphrey, James Huntley, Janet K. Jansson, Rob Knight, Chris L. Lauber, Catherine A. Lozupone, Sean McNally, David M. Needham, Sarah M. Owens, Alma E. Parada, Rachel Parsons, Geoff Smith, Luke R. Thompson, Luke Thompson, Peter J. Turnbaugh, William A. Walters, Laura Weber

Group: Earth Microbiome Project

Summary: The 16S protocol detailed here is designed to amplify prokaryotes (bacteria and archaea) using paired-end 16S community sequencing on the Illumina platform. Primers 515F-806R target the V4 region of the 16S SSU rRNA.For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. (2012). You will need to make your sample more complex by adding 5-10% PhiX to your run.

Associated Publications: Ul-Hasan S, Bowers RM, Figueroa-Montiel A, Licea-Navarro AF, Beman JM, Woyke T, Nobile CJ (2019) Community ecology across bacteria, archaea and microbial eukaryotes in the sediment and seawater of coastal Puerto Nuevo, Baja California. PLoS ONE 14(2): e0212355. doi: 10.1371/journal.pone.0212355

Affiliations: EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium

External URL: http://press.igsb.anl.gov/earthmicrobiome/protocols-and-standards/16s/

Version: 1

Publication Date: 2018

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