Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Ben Humphreys, Yuhei Kirita 2018. Nuclei isolation from human kidney for single-nucleus RNA-seq. protocols.io https://dx.doi.org/10.17504/protocols.io.nahdab6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.nahdab6
Authors: Ben Humphreys, Yuhei Kirita
Group: Human Cell Atlas Method Development Community
Summary: This protocol is based upon Habib, N et al., Nature Methods 14: 955-958, 2017 and Basu et al., Protocol Exchange 2017: DroNc-seq step-by-step with adaptions for adult human kidney including: Tissue mincing, homogenization strokes, addition of protease inhibitor and RNasin and adjustments to strainer size and sequence.
Affiliations: Washington University in St. Louis, The Humphreys Lab
Version: 1
Publication Date: 2018
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
