Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/DOI:10.17504/protocols.io.mwnc7de

Authors: Collin Closek, Anni Djurhuus, Katie Pitz, Ryan Kelly, Reiko Michisaki, Kristine Walz, Hilary Starks, Francisco Chavez, Alexandria Boehm, Mya Breitbart

Group: MBON eDNA

Summary: This protocol is aimed at amplifying the cytochrome c oxidase subunit I (COI) mitochondrial gene in eukaryotes. The primers (forward: mlCOIintF, reverse: HCO2198) utilized in this protocol are based on the primers utilized in Leray et al. 2013 (forward) and Folmer et al. 1994 (reverse).Amplicons generated using this protocol can then be sequenced using the Illumina platform.Primers used:Fluidigm CS1+mlCOIinfFFluidigm CS2+HCO2198Secondary COI PCR primersPE1-BC-CS1PE2-BC-CS2

Affiliations: Center for Ocean Solutions, Stanford University, CA, University of South Florida, College of Marine Science, St Petersburg, FL, Monterey Bay Aquarium Research Institute, Moss Landing, CA, University of Washington, Seattle, WA, Monterey Bay Aquarium Research Institute, Moss Landing, CA, Monterey Bay Aquarium Research Institute, Moss Landing, CA, Center for Ocean Solutions, Stanford University, CA, Monterey Bay Aquarium Research Institute, Moss Landing, CA, Center for Ocean Solutions, Stanford University, CA, University of South Florida, College of Marine Science, St Petersburg, FL

Version: 1

Publication Date: 2018

Expand All Collapse