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URL: https://dx.doi.org/DOI:10.17504/protocols.io.mc7c2zn

Authors: Anna Lyubimova, Shalev Itzkovitz, Jan Philipp Junker, Zi Peng Fan, Xuebing Wu, Alexander van Oudenaarden

Group: Human Cell Atlas Method Development Community

Summary: We present a protocol for visualizing and quantifying single mRNA molecules in mammalian (mouse and human) tissues. In the approach described here, sets of about 50 short oligonucleotides, each labeled with a single fluorophore, are hybridized to target mRNAs in tissue sections. Each set binds to a single mRNA molecule and can be detected by fluorescence microscopy as a diffraction-limited spot. Tissue architecture is then assessed by counterstaining the sections with DNA dye (DAPI), and cell borders can be visualized with a dye-coupled antibody. Spots are detected automatically with custom-made software, which we make freely available. The mRNA molecules thus detected are assigned to single cells within a tissue semiautomatically by using a graphical user interface developed in our laboratory. In this protocol, we describe an example of quantitative analysis of mRNA levels and localization in mouse small intestine. The procedure (from tissue dissection to obtaining data sets) takes 3 d. Data analysis will require an additional 3–7 d, depending on the type of analysis.

Affiliations: Massachusetts Institute of Technology, Hubrecht Institute—KNAW, Massachusetts Institute of Technology, Weizmann Institute of Science, Massachusetts Institute of Technology, Hubrecht Institute—KNAW, Massachusetts Institute of Technology, Massachusetts Institute of Technology, Massachusetts Institute of Technology

External URL: http://www.nature.com/doifinder/10.1038/nprot.2013.109

Version: 1

Publication Date: 2018

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