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Protocol Name
PCR partial Control Region Chaetophractus 456 bp
DOI:DOI:10.17504/protocols.io.kmncu5e RRID Copied  
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Sebastian Poljak 2017. PCR partial Control Region Chaetophractus 456 bp. protocols.io https://dx.doi.org/10.17504/protocols.io.kmncu5e
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.kmncu5e

Authors: Sebastian Poljak

Summary: We report a phylogeographic study of Chaetophractus villosus populations in Argentina. Control Region (CR) sequences (484 bp) were obtained for 76 C. villosus from 20 locations across the species whole distribution range. Seventeen new haplotypes were identified. The highest genetic variation and the earliest fossils were found in the Pampean Region, thus appearing as the most probable area of origin of the species. A general pattern of Contiguous Range Expansion (CRE) was revealed by Nested Clade Analysis (NCA) supported by mismatch analysis and Fu’s test. The Pampean Region would have been the preexpansion area, while Patagonia would have been the main dispersal route of contiguous expansion, possibly after the Pleistocenic glaciations.

Affiliations: CADIC-CONICET Molecular Ecology Lab., Bernardo Houssay 200 Ushuaia, Argentina

External URL: http://digital.bl.fcen.uba.ar/Download/paper/paper_10557903_v55_n1_p38_Poljak.pdf

Version: 2

Publication Date: 2017

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