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URL: https://dx.doi.org/DOI:10.17504/protocols.io.kkqcuvw

Authors: Daubian Santos, Guilherme Cunha Ribeiro, Aline Diniz Cabral, Márcia Aparecida Sperança

Summary: Preparation of insect specimens for morphological studies classically employs chaotropic salts which can cause disruption of important structures. Also, extraction of nucleic acids for genetic studies leads to destruction of the specimen. Thus, in this paper is proposed a new technique, based on the use of proteinase K, which allows DNA extraction while keeping intact the entire morphology of insect specimens. The presented technique can contribute to taxonomic and systematic studies on different groups of arthropods. Also, with the global emergency and reemergence of diseases transmitted by arthropod vectors, genetic and morphological investigations performed in an individual-scale would unveil important aspects of environment and host-pathogen interactions.

Associated Publications: Santos D, Ribeiro GC, Cabral AD, Sperança MA (2018) A non-destructive enzymatic method to extract DNA from arthropod specimens: Implications for morphological and molecular studies. PLoS ONE 13(2): e0192200. doi: 10.1371/journal.pone.0192200

Affiliations: Universidade Federal do ABC, Universidade Federal do ABC, Universidade Federal do ABC, Universidade Federal do ABC

External URL: https://doi.org/10.1371/journal.pone.0192200

Version: 1

Publication Date: 2018

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