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Protocol Name
DOI:DOI:10.17504/protocols.io.kcicsue RRID Copied  
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Michiyo Tsuru 2018. Culture of bone marrow stromal cells and mesenchymal stem cells. protocols.io https://dx.doi.org/10.17504/protocols.io.kcicsue
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.kcicsue

Authors: Michiyo Tsuru

Group: Clinical Proteomics and Gene Therapy Laboratory

Summary: A bone marrow aspirate was obtained from equine ilium, and mesenchymal stem cells (MSCs) were separated from the aspirate by density-gradient centrifugation (Ficoll-Hypaque gradient centrifugation) (GE Healthcare, Pittsburgh, PA, USA) and further cultured.

Associated Publications: Tsuru M, Ono A, Umeyama H, Takeuchi M, Nagata K (2018) Ubiquitin-dependent proteolysis of CXCL7 leads to posterior longitudinal ligament ossification. PLoS ONE 13(5): e0196204. doi: 10.1371/journal.pone.0196204

Affiliations: Clinical Proteomics and Gene Therapy

External URL: https://doi.org/10.1371/journal.pone.0196204

Version: 1

Publication Date: 2018

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