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URL: https://dx.doi.org/DOI:10.17504/protocols.io.kbfcsjn

Authors: Bianca Hemmeryckx, Dries Bauters, H. Roger Lijnen

Summary: Increasing energy expenditure by stimulating thermogenesis through activation of brown adipose tissue (BAT) and/or induction of browning of white adipose tissue (WAT) is considered a promising strategy to treat/prevent obesity and related metabolic diseases. Whereas WAT is adapted to store energy as triglycerides, BAT produces heat (non-shivering thermogenesis). In brown adipocytes, the uncoupling protein-1 (Ucp-1) regulates conversion of energy into heat by uncoupling ATP production from mitochondrial respiration. Also in WAT adaptive Ucp-1 positive adipocytes (brown in white: brite or beige) can arise, predominantly in subcutaneous (s) WAT. Therefore, this browning of sWAT can be visualized by making sWAT protein extracts and by analyzing protein levels of brown adipocyte-specific markers such as Ucp-1 and peroxisome proliferator-activated receptor gamma coactivator 1 α (Pgc1α) in these extracts. 

Associated Publications: Bauters D, Bedossa P, Lijnen HR, Hemmeryckx B (2018) Functional role of ADAMTS5 in adiposity and metabolic health. PLoS ONE 13(1): e0190595. doi: 10.1371/journal.pone.0190595

Affiliations: KU Leuven, KU Leuven, KU Leuven

External URL: https://doi.org/10.1371/journal.pone.0190595

Version: 1

Publication Date: 2018

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