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URL: https://dx.doi.org/DOI:10.17504/protocols.io.k5ecy3e

Authors: Carolyn Elya, Ciera Martinez

Summary: Paraffin embedding and sectioning is a classic technique used in a variety of biological disciplines. Though the fruit fly Drosophila melanogaster are one of the most commonly-employed model organisms, the immense Drosophila literature is lacking in current protocols for sectioning whole, adult flies. Here, we adopted a plant histology protocol to embed entire adult flies for thin sectioning and present our protocol for performing FISH on the subsequent sections. In the interest of reproducibility, we have tried to include details that are normally omitted from published protocols.

Associated Publications: Elya C, Lok TC, Spencer QE, McCausland H, Martinez CC, Eisen M, Robust manipulation of the behavior of by a fungal pathogen in the laboratory. eLife doi: 10.7554/eLife.34414

Affiliations: Department of Molecular and Cell Biology, University of California, Berkeley, CA, Department of Molecular and Cell Biology, University of California, Berkeley, CA

External URL: https://doi.org/10.7554/eLife.34414

Version: 1

Publication Date: 2017

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