Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/DOI:10.17504/protocols.io.jytcpwn

Authors: Carine Puppo, Tomas Voisin, Brigitte Gontero

Group: High molecular weight DNA extraction from all kingdoms

Summary: Asterionella formosa is a freshwater pennate diatom that forms star-shaped colonies, whose mitochondrial DNA was recently sequenced [1]. This diatom is of interest for genetic studies [2,3] and for the study of photosynthesis [4,5]. However, extraction of genomic DNA (gDNA) from A. formosa is difficult because of its silica cell-wall and the presence of photosynthetic pigments that contaminate the DNA. Here, we present an optimised protocol for gDNA extraction from A. formosa that overcomes those two problems. We also assessed its efficacity in yielding high-purity gDNA compared to a standard protocol using hexadecyltrimethylammonium bromide (CTAB).References[1] Villain A. et al. (2017) Mitochondr. DNA. 2 (1), 97-98.[2] De Bruin A. et al. (2004) J. Phycol. 40, 823-830.[3] van Den Wyngaert S. et al. (2015) Mol. Ecol. 24, 2955-2972.[4] Boggetto N. et al. (2007) J. Phycol. 43, 1227-1235.[5] Erales J. et al. (2008) J. Phycol. 44, 1455-1464.

Affiliations: Aix Marseille Univ CNRS, BIP, Imperial College of London, Aix Marseille Univ CNRS, BIP

Version: 1

Publication Date: 2017

Expand All Collapse