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Isaac Núñez, Tamara Matute 2017. Colony mix assay. protocols.io https://dx.doi.org/10.17504/protocols.io.jsucnewCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.jsucnew
Authors: Isaac Núñez, Tamara Matute
Group: Laboratorio de Tecnologias Libres
Summary: Protocol to co-culture individual colonies of different strains (e.g. transformed with different vectors) on a LB agar plate in a distributed and random manner.This assay has been used for low cost and open source fluorescence imaging (please see https://osf.io/dy6p2/ for further information & data, https://github.com/SynBioUC/FluoPi for code and http://docubricks.com/viewer.jsp? for hardware assembly)
Associated Publications: Nuñez I, Matute T, Herrera R, Keymer J, Marzullo T, Rudge T, Federici F (2017) Low cost and open source multi-fluorescence imaging system for teaching and research in biology and bioengineering. PLoS ONE 12(11): e0187163. doi: 10.1371/journal.pone.0187163
Affiliations: Pontificia Universidad Católica de Chile, Pontificia Universidad Católica de Chile
External URL: https://doi.org/10.1371/journal.pone.0187163
Version: 2
Publication Date: 2017
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