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Chise Suzuki, Reiji Aoki, Ayako Aoki-Yoshida 2017. Immunostaining of the isolated lymphoid follicles (ILFs) in the whole small intestine. protocols.io https://dx.doi.org/10.17504/protocols.io.jr3cm8nCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.jr3cm8n
Authors: Chise Suzuki, Reiji Aoki, Ayako Aoki-Yoshida
Summary: The immunostaining detection of isolated lymphoid follicles (ILFs) was performed according to the method of McDonald and Newberry [BioTechniques. 2007; 43(1): 50-56], with some modifications. Instead of mounting the tissue samples on a mounting plate, each segment was treated in a 15 ml tube in each step. DAB stained intestines were finally mounted on a glass slide using an aqueous mounting medium.
Associated Publications: Suzuki C, Aoki-Yoshida A, Aoki R, Sasaki K, Takayama Y, Mizumachi K (2017) The distinct effects of orally administered Lactobacillus rhamnosus GG and Lactococcus lactis subsp. lactis C59 on gene expression in the murine small intestine. PLoS ONE 12(12): e0188985. doi: 10.1371/journal.pone.0188985
Affiliations: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), The University of Tokyo
External URL: https://doi.org/10.1371/journal.pone.0188985
Version: 2
Publication Date: 2017
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