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Catherine C. Bannon, Douglas A. Campbell 2017. Phytoplankton sinking rates through time-resolved fluorescence plate spectroscopy. protocols.io https://dx.doi.org/10.17504/protocols.io.jqzcmx6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.jqzcmx6
Authors: Catherine C. Bannon, Douglas A. Campbell
Summary: We established a method for parallel, large scale analysis of multiple phytoplankton sinking rates through top-view monitoring of chlorophyll a fluorescence in microtitre well plates. We verified the method through experimental analysis of known factors that influence sinking rates, including exponential versus stationary growth phase in species of different cell sizes; Thalassiosira pseudonana CCMP1335, chain-forming Skeletonema marinoi RO5A and Coscinodiscus radiatus CCMP312. We fit decay curves to an algebraic transform of the decrease in fluorescence signal as cells sank away from the fluorometer detector, and then used minimal mechanistic assumptions to extract a sinking rate (m d-1) using an RStudio script, SinkWORX.
Affiliations: Mount Allison University, Mount Allison University
External URL: https://www.dropbox.com/sh/w03c2nt97rjk990/AACyg_nJrl75ztT9s6A89zT-a?dl=0
Version: 1
Publication Date: 2017
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