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Mia Maltz, Allison SD, German D, Looby C. 2017. Extracellular enzyme assays (NAG, BG, and CBH). protocols.io https://dx.doi.org/10.17504/protocols.io.jf8cjrwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.jf8cjrw
Authors: Mia Maltz, Allison SD, German D, Looby C.
Summary: We conducted fluorimetric assays for each of three hydrolytic enzymes: b-glucosidase and cellobiohydrolase, and N-acetyl-glucosaminidase in black microplates. We measured fluorescence at 365 nm excitation and 450 nm emission. From each sample, we recorded fluorescence values for MUB substrate (substrate control), homogenate (homogenate control), MUB standards in the presence of maleate buffer (standard), and MUB in the presence of homogenate. We calculated potential extracellular enzyme activity of NAG, BG, and CBH from each litter sample.
Affiliations: UC Riverside, UC Irvine, UC Irvine, UC Irvine
Version: 1
Publication Date: 2017
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