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Jernej Turnsek 2017. HA tag enables highly efficient detection of heterologous proteins in Phaeodactylum tricornutum (Pt) exconjugants. protocols.io https://dx.doi.org/10.17504/protocols.io.j7ncrmeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.j7ncrme
Authors: Jernej Turnsek
Group: Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: Invitrogen's HA tag monoclonal antibody in combination with Western Breeze Anti-Mouse Chemiluminescent Western Blot Immunodetection Kit leads to no background Western blots with Pt lysates. HA tag thus represents a tag of choice for (1) efficient and unambioguous protein expression analysis, (2) immunofluorescence experiments and (3) various IP and pull-down experiments in Pt and possibly other diatoms as well as other marine microeukaryotes.
Affiliations: University of California, Berkeley
Version: 2
Publication Date: 2017
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