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Protocol Name
qRT-PCR
DOI:DOI:10.17504/protocols.io.ixecfje RRID Copied  
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Ning Chen 2017. qRT-PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.ixecfje
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.ixecfje

Authors: Ning Chen

Summary: qRT- PCR for each gene was carried out using a thermal cycler (Bio-Rad, Hercules, CA, USA) and amplification conditions were 40 cycles of 30s at 95°C, 3 s at 95°C, and 30 s at 60°C.

Associated Publications: Chen N, Du B, Zhou H, Shen F, Li J, Xie Z (2017) Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. PLoS ONE 12(8): e0182773. doi: 10.1371/journal.pone.0182773

Affiliations: Women’s Hospital, School of Medicine, Zhejiang University

External URL: https://doi.org/10.1371/journal.pone.0182773

Version: 1

Publication Date: 2017

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