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Protocol Name
PD-L1 22C3 LDT on VENTANA BenchMark ULTRA automate
DOI:DOI:10.17504/protocols.io.ixacfie RRID Copied  
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Marius Ilié, Paul Hofman 2017. PD-L1 22C3 LDT on VENTANA BenchMark ULTRA automate. protocols.io https://dx.doi.org/10.17504/protocols.io.ixacfie
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.ixacfie

Authors: Marius Ilié, Paul Hofman

Summary: Optimised VENTANA BenchMark ULTRA protocol for PD-L1 assay using the concentrate 22C3 antibody.For the purpose of protocol development 95% of clinical specimens consisted of surgical resection specimens and the remaining 5% were bronchial biopsies and transbronchial mediastinal lymph node biopsies.

Associated Publications: Ilie M, Khambata-Ford S, Copie-Bergman C, Huang L, Juco J, Hofman V, Hofman P (2017) Use of the 22C3 anti–PD-L1 antibody to determine PD-L1 expression in multiple automated immunohistochemistry platforms. PLoS ONE 12(8): e0183023. doi: 10.1371/journal.pone.0183023

Affiliations: Université Côte d'Azur, University Hospital Federation OncoAge, Laboratory of Clinical and Experimental Pathology, Hôpital Pasteur, Nice, France, Université Côte d'Azur, University Hospital Federation OncoAge, Laboratory of Clinical and Experimental Pathology, Hôpital Pasteur, Nice, France

External URL: https://doi.org/10.1371/journal.pone.0183023

Version: 1

Publication Date: 2017

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