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Protocol Name
RNA Stable Isotope Probing Experimental Set Up
DOI:DOI:10.17504/protocols.io.iuzcex6 RRID Copied  
PDF Report How to cite
Julie Huber, Caroline Fortunato 2017. RNA Stable Isotope Probing Experimental Set Up. protocols.io https://dx.doi.org/10.17504/protocols.io.iuzcex6
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.iuzcex6

Authors: Julie Huber, Caroline Fortunato

Group: Center for Dark Energy Biosphere Investigations, Huber Lab

Summary: This is a protocol for carrying out RNA Stable Isotope Probing experiments using seawater or vent fluids to examine microbial bicarbonate uptake. It was developed at Axial Seamount to examine autotrophy in deep-sea hydrothermal vent fluids. We have used modified versions in seawater, cold crustal environments, and other hydrothermal vents as well.

Affiliations: WHOI, WHOI

Version: 2

Publication Date: 2017

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