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Dr. Daniel Richter 2017. TruSeq RNA Preparation with Modification for Four Rounds of Poly-A Selection. protocols.io https://dx.doi.org/10.17504/protocols.io.imucc6wCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.imucc6w
Authors: Dr. Daniel Richter
Group: Ecology of Marine Plankton (ECOMAP) team - Roscoff, Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: March, 2012; based on sample preparation guide v2, catalog # RS-930-1021, part # 15026495 Rev. A, August 2011Modifications: samples in PCR tubes (because a plate/seal will not be used, some numbered steps are skipped); include two rounds of poly-A selection (with two separate sets of beads); repeat clean-up step after PCR to remove additional adapters; elute with 1.5 ul less volume off of beads (to leave 1 ul behind, not 2.5 ul)
Associated Publications: Richter, Daniel J and Fozouni, Parinaz and Eisen, Michael and King, Nicole. Gene family innovation, conservation and loss on the animal stem lineage. 2018;7:e34226 https://doi.org/10.7554/eLife.34226
External URL: https://elifesciences.org/articles/34226
Version: 1
Publication Date: 2017
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