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Protocol Name
DOI:DOI:10.17504/protocols.io.i6vche6 RRID Copied  
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Miriam Schalamun 2017. High molecular weight gDNA extraction after Mayjonade et al. optimised for eucalyptus for nanopore sequencing. protocols.io https://dx.doi.org/10.17504/protocols.io.i6vche6
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.i6vche6

Authors: Miriam Schalamun

Group: High molecular weight DNA extraction from all kingdoms

Summary: Extraction of high quality DNA for long read sequencing e.g. the Oxford Nanopore Optimized for DNA extraction from eucalyptus grandis and eucalyptus pauciflora. This protocol contains an optional Chloroform clean up step which is necessary for eucalyptus but might not be for other tissue. For long DNA fragments don't vortex the DNA sample. 

Version: 7

Publication Date: 2017

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