Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/DOI:10.17504/protocols.io.i32cgqe

Authors: Lucas G. Huggins, Sheena M. Cruickshank, Richard Preziosi, Kathryn J. Else

Summary: A non-invasive PCR-based methodology for sensitive detection and identification of parasitic nematode DNA released in the faeces of infected amphibians and reptiles as egg or tissue fragments (environmental DNA). A DNA extraction protocol optimised for liberation of DNA from resilient parasite eggs was developed alongside the design of a novel, nematode universal, degenerate primer pair that has broad-spectrum detection ability for shed nematode eDNA. 

Associated Publications: Huggins LG, Michaels CJ, Cruickshank SM, Preziosi RF, Else KJ (2017) A novel copro-diagnostic molecular method for qualitative detection and identification of parasitic nematodes in amphibians and reptiles. PLoS ONE 12(9): e0185151. doi: 10.1371/journal.pone.0185151

Affiliations: University of Manchester, University of Manchester, Manchester Metropolitan University, University of Manchester

External URL: https://doi.org/10.1371/journal.pone.0185151

Version: 1

Publication Date: 2017

Expand All Collapse