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URL: https://dx.doi.org/DOI:10.17504/protocols.io.hvpb65n

Authors: Kelsey Miller

Group: BioLegend

Summary: This protocol is essentially the same as a standard ELISA protocol, with one major exception – all animal compounds must be avoided in buffers, etc. because they contain Neu5Gc, which will interfere with antibody activity and result in misleading data. Essentially, the glycoconjugate to be evaluated is immobilized on an ELISA plate and exposed to a primary antibody. The antibody provided in this kit has been shown to identify as little as 5 pmol of Neu5Gc per microgram glycoprotein, which is at or below the current detection limit for conventional DMB HPLC analysis. An advantage of the ELISA procedure is that it confirms the presence of Neu5Gc on the glycoconjugate of interest, but use caution, as it also reacts with solution/media that contains glycoconjugate.

Affiliations: BioLegend

External URL: http://www.biolegend.com/media_assets/support_protocol/Neu5Gc_Antibody_Kit_Protocols_110813.pdf

Version: 2

Publication Date: 2017

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