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Philip D. Weyman 2017. Rolling circle amplification-mediated hairpin (hp)-RNA libraries. protocols.io https://dx.doi.org/10.17504/protocols.io.hnrb5d6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.hnrb5d6
Authors: Philip D. Weyman
Group: Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: A sequence-independent method to turn genomic DNA into libraries of hairpins. An efficient way to get a different adapter on each end of a DNA fragment is to clone it and digest the fragment back out. Design the vector to have unique restriction sites outside the unique regions you want to put on each end. After digestion, your DNA fragments will have two different "adapters" on each end.Adapted from: Wang L., Zheng J., Luo Y., Xu T., Zhang Q., Zhang L., Xu M., Wan J., Wang MB., Zhang C., Fan Y. 2013. Construction of a genomewide RNAi mutant library in rice. Plant Biotechnology Journal 11:997–1005. DOI: 10.1111/pbi.12093.
Affiliations: J. Craig Venter Institute
Version: 1
Publication Date: 2017
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