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Protocol Name
Concentrating Viruses by Tangential Flow Filtration
DOI:DOI:10.17504/protocols.io.hgrb3v6 RRID Copied  
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Dr. Steven Wilhelm,  Samantha Coy 2017. Concentrating Viruses by Tangential Flow Filtration. protocols.io https://dx.doi.org/10.17504/protocols.io.hgrb3v6
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.hgrb3v6

Authors: Dr. Steven Wilhelm, Samantha Coy

Group: VERVE Net, The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols

Summary: Ultracentrifugation in the WX Ultra Series can support only 30 mL samples at a time, and therefore do not significantly concentrate viruses. Tangential flow filtration enables viral concentration of large volumes that can then be purified using sucrose density gradients. Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.

Affiliations: ,

Version: 2

Publication Date: 2017

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