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Mariana Rius, Joshua Rest 2017. Genome-wide Kozak Sequence Free Energy Analysis. protocols.io https://dx.doi.org/10.17504/protocols.io.h9mb946Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.h9mb946
Authors: Mariana Rius, Joshua Rest
Group: Protist Research to Optimize Tools in Genetics (PROT-G), Collier Lab
Summary: The purpose of this protocol is to evaluate the significance of delta G values, as a strategy to determine the significance of secondary structure. Sequences are shuffled randomly 100x, maintaining base composition but not base order. The percentile of the delta G vlaue of the non-scrambled (original) sequence is obtained from the distribution created by the delta G values of the 100X scrambled sequences.
Affiliations: Stony Brook University, Stony Brook University
Version: 1
Publication Date: 2017
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