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URL: https://dx.doi.org/DOI:10.17504/protocols.io.gvnbw5e

Authors: LI-COR Biosciences

Group: LI-COR Biosciences

Summary: Invitrogen’s patented Syto dyes are cell-permeant cyanine dyes that bind to nucleic acids. Several Syto dyes are available with varying cell permeability, fluorescence enhancement upon binding to nucleic acids, excitation and emission spectra, and nucleic acid selectivity and binding affinity. The Syto 60 stain has absorption and fluorescence emission maxima of 652/678 nm. Nucleic acids stained with the Syto 60 stain can be detected and quantified on the Odyssey® Infrared and Odyssey Fc Imaging Systems using the 700 nm channel. In the procedures outlined, the Syto 60 dye was used to stain serial dilutions of a 1 kb DNA ladder and a 50 bp DNA ladder (New England Biolabs, P/N N3232 and N3236, respectively). Three methods are presented for staining of DNA in this technical note. The Syto 60 stain can be included in the DNA sample for detection using an Odyssey Imaging system.The Syto 60 stain can also be combined with ethidium bromide (EtBr) and included in the DNA sample for visualization on an Odyssey Imaging System and on a UV transilluminator; or the Syto 60 stain can be diluted and used alone as a post-electrophoresis gel stain. For additional information, see Imaging Nucleic Acid Gels on the Odyssey Fc Imager.

Affiliations: LI-COR Biosciences

External URL: https://www.licor.com/documents/eo6n96n0kg8hfgc50dutu73byr0heg4r

Version: 1

Publication Date: 2018

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