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Amy Zimmerman 2017. Propagation of marine eukaryotic viruses (Prasinoviruses). protocols.io https://dx.doi.org/10.17504/protocols.io.gujbwunCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.gujbwun
Authors: Amy Zimmerman
Group: VERVE Net, Worden Lab
Summary: Purpose: To reproducibly generate fresh preparations of virus for independent experiments.Fresh virus sample for an experiment is generated by a primary infection of exponentially growing host cells from a master stock of virus. The infected host is allowed to lyse until the culture is cleared. The lysate is filtered to remove any large cellular debris, then the viral-size fraction is concentrated from the filtered lysate and washed with buffer using a centrifugal concentrator. The viral concentrate is stored at 4°C protected from light and should be used for an experiment within 1-2 days. On the day of (or the day before) the experiment, an MPN assay should be set up to assess infectivity of the fresh viral concentrate.
Affiliations: Worden Lab, Monterey Bay Aquarium Research Institute
Version: 2
Publication Date: 2017
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