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Amy Zimmerman, Camille Poirier 2017. Enumerating algal viruses by flow cytometry. protocols.io https://dx.doi.org/10.17504/protocols.io.guibwueCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.guibwue
Authors: Amy Zimmerman, Camille Poirier
Group: VERVE Net, Worden Lab
Summary: Purpose: To quantify virus-like particles in culture or environmental samples.Host cells and virus-like particles are enumerated from the same glutaraldehyde-fixed sample. For virus counts, an aliquot is diluted in 0.02-µm-filtered TE buffer prior to staining with SYBR Green I nucleic acid dye to improve staining efficiency and ensure an appropriately low event rate in the instrument. An unstained aliquot of sample is run for host cell counts.
Affiliations: Worden Lab, Monterey Bay Aquarium Research Institute, Worden Lab, Monterey Bay Aquarium Research Institute
Version: 2
Publication Date: 2017
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