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Anna Behle 2017. Isolation of plasmid DNA from E. coli (Alkaline lysis method). protocols.io https://dx.doi.org/10.17504/protocols.io.gtbbwinCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.gtbbwin
Authors: Anna Behle
Group: Axmann Lab
Summary: Isolation of plasmid DNA from E. coli using the alkaline lysis method modified from Birnboim et al., 1979. This protocol is suitable for fast, cheap recovery of large amounts of plasmid, e.g. for cloning purposes or restriction analysis. However, the purity of plasmid is insufficient for sequencing.
Affiliations: Institute for Synthetic Microbiology, Heinrich-Heine-University, Düsseldorf
Version: 1
Publication Date: 2017
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