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Daniel Vaulot 2016. Tangential flow filtration (TFF) concentration of phytoplankton. protocols.io https://dx.doi.org/10.17504/protocols.io.gpybvpwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.gpybvpw
Authors: Daniel Vaulot
Group: Ecology of Marine Plankton (ECOMAP) team - Roscoff, Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: Concentrate phytoplankton samples about 100-fold typically from 5L down to 20 mL. Takes about 1 hour per sample. Samples can be used for flow cytometry sorting or for cultures. Enrichment by TFF usually keep growing for a longer time than unconcentrate samples.ReferenceMarie, D., Shi, X.L., Rigaut-Jalabert, F. & Vaulot, D. (2010). Use of flow cytometric sorting to better assess the diversity of small photosynthetic eukaryotes in the English Channel. FEMS Microbiology Ecology. 72. p.pp. 165–178.
Affiliations: Station Biologique, Roscoff, France
Version: 2
Publication Date: 2016
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