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April Woods, G Jason Smith 2017. Modified Bacterial Conjugation Protocol For Pseudo-nitzschia multiseries. protocols.io https://dx.doi.org/10.17504/protocols.io.gj6bureCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.gj6bure
Authors: April Woods, G Jason Smith
Group: Protist Research to Optimize Tools in Genetics (PROT-G), Environmental Biotechnology Lab
Summary: This is a modified version of 'Conjugation of Thalassiosira pseudonana', publishedby J. Turnsek dx.doi.org/10.17504/protocols.io.f55bq86 .The protocol was modified to enhance viability in the diatom Pseudo-nitzschia multiseries. This version was used to transform Pseudo-nitzschia multiseries isolate 15091C3 with an episomal plasmid derived from pPtPUC3 to express a egfp gene under control of the P. multiseries actin promoter and termination domains.
Affiliations: Moss Landing Marine Labs, Moss Landing Marine Labs
Version: 1
Publication Date: 2017
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