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Protocol Name
SensiFAST™ HRM Kit
DOI:DOI:10.17504/protocols.io.fyrbpv6 RRID Copied  
PDF Report How to cite
Bioline 2016. SensiFAST™ HRM Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.fyrbpv6
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.fyrbpv6

Authors: Bioline

Group: Bioline

Summary: The SensiFAST™ HRM Kit has been developed for fast, highly reproducible High Resolution Melt (HRM) analysis and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST HRM Kit delivers fast, highly-specific and ultra-sensitive HRM analysis. For ease-of-use and added convenience, SensiFAST HRM is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the EvaGreen® dye, dNTPs, stabilisers and enhancers. As a ready-to-use premix, only primers and template need to be added.

External URL: http://www.bioline.com/us/downloads/dl/file/id/2705/sensifast_hrm_kit_manual.pdf

Version: 1

Publication Date: 2016

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