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URL: https://dx.doi.org/DOI:10.17504/protocols.io.frjbm4n

Authors: New England BioLabs, Inc.

Group: New England Biolabs (NEB)

Summary: Cas9 nuclease may be used in vivo to create targeted genome modifications.  There are several ways in which to introduce Cas9-guide RNA complexes into cells.  Here we present a method for the transfection of Cas9 RNP’s into HEK293 FT cells using Thermo Fisher Lipofectamine® RNAiMAX.  This is a ‘reverse transfection’ method that uses a final concentration of 10 nM RNP per transfection in a 96-well culture plate.

Affiliations: ,

External URL: https://www.neb.com/protocols/2016/07/26/transfection-of-cas9-rnp-ribonucleoprotein-into-adherent-cells-using-the-lipofectamine-rnaimax

Version: 1

Publication Date: 2016

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