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Sarah Giuliani 2016. Sampling for RNA / Protein/ DOM/ PO4: Large-scale One-step Phage Infection of Cyanobacteria. protocols.io https://dx.doi.org/10.17504/protocols.io.fkvbkw6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.fkvbkw6
Authors: Sarah Giuliani
Group: Coleman Lab
Summary: Experiment purpose is to monitor the time-course of a large-scale infection of host cyanobacteria by phage under variable media conditions and obtain samples for proteomic and transcriptomic analysis.9 Hourly Timepoints: 0, 1, 2, 3, 4, 6, 8, 10, 14Sampling is to collect supernatant fractions to determine total dissolved phosphate and soluble reactive phosphate (TDP/SRP) for analysis of inorganic phosphate concentration and phosphate in dissolved organic material (DOM); and to collect pellets to isolate RNA for transcriptomics and proteins for proteomics.
Affiliations: Coleman Lab - University of Chicago, Department of the Geophysical Sciences
Version: 1
Publication Date: 2016
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