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Ben Tully 2016. ECOGEO 'Omics Training: 2.0 Quality Control. protocols.io https://dx.doi.org/10.17504/protocols.io.fixbkfnCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.fixbkfn
Authors: Ben Tully
Group: ECOGEO
Summary: Quality impact results is the universal first step for all sequencing methods. This protocol is for sequencing results in FASTQ format. FastQC allows for visualization of quality scores. Sequence trimming allows for removal of residual primer sequences and increases overall quality scores. Trimmomatic does both. CutAdapt cuts adapters from sequences. Sickle creates a sliding window for quality and length trimming of FASTQ files.Open this protocol inside the virtual machine (details in 'Start Instructions') for easy copy, paste of commands into the command line terminal window.
Affiliations: EarthCube Oceanography and Geobiology Environmental 'Omics
Version: 2
Publication Date: 2016
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