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Ramon terrado 2016. Isotopic labeling of freshwater mixotrophic algae using isotopic labeled heat-killed bacteria. protocols.io https://dx.doi.org/10.17504/protocols.io.ezdbf26Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.ezdbf26
Authors: Ramon terrado
Group: Caron Lab - Protistan Ecology
Summary: This protocol aims to achieve an isotopic labeling of mixotrophic algae using 15N and 13C labeled heat-killed bacteria. It was developped to be used with the mixorophic chrysophyte Ochromonas to asses the sources of carbon and nitrogen of this algae when growing mixotrophically . Sources of nitrogen and carbon in the media used were reduced to 1 inorganic source (NH4Cl for nitrogen and bicarbonate for carbon) and 1 organic source (heat-killed bacteria); media used for Ochromonas growth was modified accordingly to reflect this.Downstream analysis of isotopic labeled Ochromonas include bulk IRMS measurements or nanoSIMS measurements.
Affiliations: University of Southern California (USC)
External URL: https://ncma.bigelow.org/media/pdf/NCMA-algal-medium-DY-V.pdf
Version: 1
Publication Date: 2016
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