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Yiheng Hu 2016. High quality DNA extraction from Fungi_small scale. protocols.io https://dx.doi.org/10.17504/protocols.io.exmbfk6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.exmbfk6
Authors: Yiheng Hu
Group: High molecular weight DNA extraction from all kingdoms, Team Schwessinger
Summary: Modified from Benjamin Schwessinger: High quality DNA from Fungi for long read sequencing e.g. PacBio. protocols.ioOptimized for DNA extraction from wheat stripe rust spores.Buffers are best when fresh and not older than 3-6 months. Buffered Phenol:Chloroform:Isoamylalcohol (25:24:1) should not be older than 3 months.Critical steps to obtain high quality DNA:Do NOT heat samples during DNA extractions! Perform all steps at RT or 4oC as indicated.Do NOT incubate samples with KAc for prolonged time periods
Version: 1
Publication Date: 2016
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