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Dave Lunt 2016. Transformation of supercompetent cells. protocols.io https://dx.doi.org/10.17504/protocols.io.etubenwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.etubenw
Authors: Dave Lunt
Group: EvoHull
Summary: Protocol to prepare E. coli supercompetent cells to transform with plasmid/ligation. This protocol is originally derived from Hanahan, D. (1983) J. Mol. Biol. 166:557-580 with some changes. This version works exceptionally well for cloning PCR products where the number of colonies is not expected to be as high as from some ligations. These cells outperform commercially purchased supercompetent cells in my hands.
Affiliations: University of Hull, UK
Version: 2
Publication Date: 2016
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