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G-Biosciences 2016. Generating CB-X™ Tables and standard curves for CB-X Protein Assay Optimization. protocols.io https://dx.doi.org/10.17504/protocols.io.e4bbgsnCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.e4bbgsn
Authors: G-Biosciences
Group: G-Biosciences
Summary: For routine protein assays, we recommend that researchers generate their own CB-X™ Tables or standard curves. This is a one time commitment to ensure user specific results and allows single tube assays to be performed each time as opposed to generating new calibration plots for each assay. We recommend using a protein standard similar to you protein of interest or a purified source of your protein.The supplied CB-X™ tables may result in some inconsistencies due to the type of cuvettes or microtiter plates used. CB-X™ Table for Spectrophotometer readings were measured with 1cm path length cuvettes against deionized water and CB-X™ Table for Microplate Reader readings were measured using Nunc™ Immuno 96 MicroWell™ Plates (MaxiSorp™ ) [Cat# 442404] against deionized water.
External URL: http://www.gbiosciences.com/PDF/Protocol/CB-X_Protein_Assay.pdf
Version: 1
Publication Date: 2016
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