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URL: https://dx.doi.org/DOI:10.17504/protocols.io.dqg5tv

Authors: Mathias Middelboe, Amy M. Chan, and Sif K. Bertelsen

Group: VERVE Net, Suttle Laboratory of Marine Molecular Microbiology and Virology

Summary: More than 40 years ago, Safferman and Morris (1963) used the plaque assay method to isolate the first cyanophage that infects a freshwater filamentous cyanobacterium, Plectonema boryanum. Since then, this approach has been used successfully to detect and isolate a number of different phages infecting marine Synechoccocus and Prochlorococcus (e.g., Suttle and Chan 1993; Waterbury and Valois 1993; Wilson et al. 1993; Sullivan et al. 2003).The advantages of this method are that results are easily interpreted as plaques formed on pigmented lawns can be easily identified. Since a plaque is the result of a single infection event, the virus can be easily purified and cloned. The first part of the procedures described as follows: (1) preparation of base plates and (2) preparation of top agar/agarose are applicable also for bacteriophage plaque assays.

Affiliations: Manual of Aquatic Viral Ecology, Manual of Aquatic Viral Ecology, Manual of Aquatic Viral Ecology

External URL: http://www.aslo.org/books/mave/MAVE_118.pdf

Version: 1

Publication Date: 2016

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