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Sonia Hall 2015. Pollak Trichrome Solution. protocols.io https://dx.doi.org/10.17504/protocols.io.dh238dCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.dh238d
Authors: Sonia Hall
Summary: Combine:150mls Ethanol150mls distilled water3mls Glacial Acetic AcidSplit into 4 beakers (approx 75mls each) and add a stir bar.Beaker 1 - Add: 0.5g Acid Fuchsin and 1.0g Ponceau 2RBeaker 2 - Add: 0.45g Light green, SF, yellowishBeaker 3 - Add: 0.75g Orange G and 1.5g Phosphotungstic acidBeaker 4 - Add: 1.5g Phosphomolybdic acidWhen each reagent is maximally dissolved, combine the 4 dyes and filter. Cover storage bottle in tin foil to reduce light exposure. Remake each month.
Affiliations: University of Massachusetts Medical School
Version: 1
Publication Date: 2015
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