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Protocol Name
bioBakery Protocols
DOI:DOI:10.17504/protocols.io.d9899v RRID Copied  
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Curtis Huttenhower 2016. bioBakery Protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.d9899v
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URL: https://dx.doi.org/DOI:10.17504/protocols.io.d9899v

Authors: Curtis Huttenhower

Group: VERVE Net, Huttenhower Lab

Summary: This tutorial focuses on performing a comprehensive metagenomic analysis from whole-genome shotgun sequencing data for microbiome studies. It is divided in to different steps that use the following metagenomic, computational tools: MetaPhlAn, GraPhlAn, LEfSe, and HUMAnN. Our last addition to the PhlAn tools is PhyloPhlAn that can be used in metagenomics for phylgenetically and taxonomically place contigs assembled from whole metagenomic sequencing samples.This tutorial is also a step-by-step description of the metagenomic pipeline we used in our review paper about computational meta'omics (specifically Figure 4).

Version: 1

Publication Date: 2016

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