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Protocol Name
Manual setting of k-mer coverage peaks
DOI:DOI:10.17504/protocols.io.d7k9kv RRID Copied  
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Afiahayati,  Sato K,  Namiki T,  Hachiya T,  Tanaka H,  Sakakibara Y. 2016. Manual setting of k-mer coverage peaks. protocols.io https://dx.doi.org/10.17504/protocols.io.d7k9kv
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.d7k9kv

Authors: Afiahayati, Sato K, Namiki T, Hachiya T, Tanaka H, Sakakibara Y.

Group: VERVE Net, Hurwitz Lab

Summary: Contrary to single-genome assemblers (e.g., Velvet and SOAPdenovo), MetaVelvet assumes metagenomics settings. Accordingly, k-mer coverage histogram might be multi-modal rather than uni-modal. Please note that the coverage peak parameters can largely affect MetaVelvet assembling results. Although simple and automatic peak detection algorithm is implemented in meta-velvetg, we strongly recommend manual inspection of k-mer coverage peaks and manual setting of the coverage peak parameters. For manually setting the coverage peak parameters, please execute the following procedures.

Affiliations: Sakakibara Lab, Sakakibara Lab, Sakakibara Lab, Sakakibara Lab, Sakakibara Lab, Sakakibara Lab

External URL: http://metavelvet.dna.bio.keio.ac.jp/MV.html

Version: 1

Publication Date: 2016

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