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URL: https://dx.doi.org/DOI:10.17504/protocols.io.d7e9jd

Authors: Vladimir Vigdorovich

Summary: This is a protocol for plasmid minipreps that does not involve any columns and uses commonly accessible reagents. While it doesn't result in highly pure DNA, it can provide a sufficient quantity of plasmid for sequencing, restriction analysis, preparative digests.Beware of the large amount of RNA that co-purifies with plasmid DNA (it acts as an efficient carrier during the nucleic acid precipitation step). This contamination will make it impossible to determine DNA concentration from standard 280-nm absorbance readings. Credits: I first learned this protocol from the old-style protocols for bacmid purification using the Bak-to-Bac system (Invitrogen). In that system, the large (>50 kb) bacmid molecules were deemed too fragile for the Qiagen spin columns.

Affiliations: Center for Infectious Disease Research

Version: 1

Publication Date: 2016

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