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Rob Ward's Lab 2015. Dissecting and Immunostaining Drosophila melanogaster Egg Chambers. protocols.io https://dx.doi.org/10.17504/protocols.io.cs9wh5Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.cs9wh5
Authors: Rob Ward's Lab
Summary: Drosophila melanogaster females' productive system has been an excellent model in studying many developmental events such as stem cell specification and cell migration. The ovaries are composed of a chain of egg chambers starting with the germaruim that buds of stage 1 egg chamber after encapsulated it with follicle cells. Then, stage 1 egg chamber goes under 14 developmental stages. This protocol provides the basic method for both dissecting and immunostaining ovaries. Each step is critical. This protocol is modified from http://www.ncbi.nlm.nih.gov/pubmed/8162854
Version: 1
Publication Date: 2015
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